PHAGE PEG precipitation purification

Phage PEG precipitation/purification
  1. Add 30 ml of phage stock to SS-Oakridge tube;
  2. Add 7,5 mL of 20% PEG-8000/2.5 M NaCl;
  3. Incubate on ice for 30 minutes or longer;
  4. Spin down phages at 11 000 rpm for 20 minutes;
  5. Respin 2-3x to remove all of PEG solution (using a micro-pipet tip facilitates removal of all solution);
  6. Resuspend phage in STE (500-1000 ┬ÁL);
  7. Transfer to Eppendorf tube and spin at 14 000 rpm for 10 minutes;
  8. Transfer the supernatant to new Eppendorf tube and label;
  9. Titer phage.
STE for 100 mL: PEG for 100 mL: