DATA Genome start determination
Due to the DNA extraction method, sequencing and assembly, it is not possible to assemble directly the genome with the correct start. Two cases are possible:
- When looking at the genome coverage, if peaks of reads are present these can be used as a signal to determine the beginning of the phage genomic sequence. This putative identification can be correlated with known similar sequences.
- If there is no clear genomic indication indicating the presence of the beginning of the genome, it might be inferred from available litterature about closest sequenced genome. If none exists, PCR and specific sequencing can enable to identify the extremity of the DNA molecule.